The invention relates to a preparation method of white carbon black/graphene oxide nano-hybrid filler, the nano-hybrid filler and application of the nano-hybrid filler. The preparation method comprises the following steps: carrying out high-speed grinding and dispersion to prepare stable white carbon black slurry modified by a silane coupling agent; then uniformly stirring and grinding and dispersing the modified white carbon black slurry with a graphene oxide/water suspension solution subjected to high-speed grinding and dispersion and/or ultrasonic treatment; connecting white black carbon and graphene oxide through a bridge effect of the silane coupling agent; and then spraying and drying to prepare the white carbon black/graphene oxide nano-hybrid filler. The nano-hybrid filler is filled into any rubber variety through a traditional melting and blending technology to prepare high-performance tire tread. The preparation method and an application technology of the nano-hybrid filler are simple; and when the nano-hybrid filler is applied to rubber, nano-dispersion of graphene in a rubber matrix can be realized and the limitation of the rubber variety can be broken through, so thata composite material has excellent mechanical property, wet slipping resistance, cutting resistance, properties of delaying dynamic and static crack growth, and the like.