The present invention provides a display substrate and a manufacturing method thereof, a display panel and a display device. The method comprises the steps of: forming at least one bonding pad moduleon a substrate, wherein each bonding pad module comprises a positive bonding pad and a negative bonding pad which are mutually insulated; forming a P-pole weld leg and an N-pole weld leg which are mutually connected on each positive bonding pad and/or negative bonding pad; printing solder paste on the positive bonding pads and the negative bonding pads; and accessing the P-pole weld legs and the N-pole weld legs on the at least one positive bonding pad and/or negative bonding pad into a closed-loop preset time frame to allow the solder paste to gathering towards the positive bonding pads and the negative bonding pads under the Lorentz force action so as to separate from the solder paste between the positive bonding pads and the negative bonding pads in each bonding pad module. Therefore, the solder paste on the positive bonding pads and the negative bonding pads is moved under the Lorentz force action so that the solder paste between the positive bonding pads and the negative bonding pads is separated to effectively avoid the solder paste short-circuit problem between the positive bonding pads and the negative bonding pads and improve the rate of finished products.