The invention discloses a ceftriaxone sodium injection which comprises ceftriaxone sodium, a liposome membrane material and D-glucopyranosylamide. The injection further comprises a fatty acid alkanolamide surfactant. In addition, the invention also discloses a preparation method of the ceftriaxone sodium injection. The injection is high in encapsulation rate and low in leakage rate, and can meet industrial requirements to a great extent; meanwhile, the blood concentration in the lung can be increased, and the treatment of respiratory tract infection diseases is facilitated.