The invention relates to a coumachlor hapten and an artificial antigen, and preparation methods and applications thereof. The structure of the coumachlor hapten is represented by formula (I); and thecoumachlor hapten artificial antigen is obtained by coupling the hapten represented by the formula (I) with a carrier protein. An animal is immunized with the coumachlor hapten artificial antigen to obtain a specific antibody with a high titer and a high sensitivity. The coumachlor hapten hapten and the prepared antibody provide a new method provide a new way for establishing a rapid, simple, inexpensive, sensitive and specific coumachlor hapten detection method.