The invention relates to an anthurium andraeanum germplasm resource in vitro preservation method. The method comprises the steps that strong suckers of anthurium andraeanum obtained after leaves are removed serve as explants, and the explants are washed out under tap water, disinfected with 72-75% alcohol on the surface, washed with sterile water once, treated with 0.1% mercury bichloride, then washed with sterile water for three times, and dried with sterile filter paper; then the explants are cut into stem segments with nodes and inoculated into a bud induction medium to be cultured for 45 days, then germinated axillary buds are transferred to a multiplication medium for expanding propagation for 40 days, and cluster seedlings are obtained; the obtained cluster seedlings are cut into individual plants and transferred into a in vitro preservation culture medium for preservation, wherein the in vitro preservation culture medium is prepared from White, 0.1-0.2 g/L of C6H5Na3O7, 30 g/L of sucrose and 5 g/L of agar powder. According to the method, test-tube plantlets of anthurium andraeanum are preserved for 16 months, botanical characteristics of progenies of the test-tube plantletsand RAPD amplification bands cannot be different, preserved materials are stable, the genetic variation cannot occur, the seed nature degeneration and virus infection can be prevented, and the excellent characteristics of germplasm can be ensured.