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发明公开
CN109932439A 一种鸟苷四磷酸的超高效液相色谱检测方法 无效 - 驳回

一种鸟苷四磷酸的超高效液相色谱检测方法
摘要：
本发明公开了属于化学分析技术领域的一种鸟苷四磷酸的超高效液相色谱检测方法。所述方法包括，采用如下色谱条件：色谱柱：反相C18色谱柱；流动相：包括流动相A与流动相B；所述流动相A为甲醇或乙腈，所述流动相B为KH2PO4溶液或K2HPO4-KH2PO4缓冲液；洗脱条件为：流动相A：流动相B＝3～10：97～90。本发明的方法能有效检测到ppGpp，基线平稳，进样量少，节约时间和成本，不需设置梯度洗脱，操作简单方便，检测效果好。
摘要（英）：

The invention discloses an ultra performance liquid chromatography detection method for guanosine tetraphosphate, which belongs to the technical field of chemical analysis. The method comprises the following chromatography conditions: a chromatography column adopts a reverse phase C18 chromatography column; mobile phases comprises a mobile phase A and a mobile phase B, the mobile phase A is methanol or acetonitrile and the mobile phase B is a KH2PO4 solution or a K2HPO4-KH2PO4 buffer, and an elution condition is that the ratio of the mobile phase A to the mobile phase B is 3-10 to 97-90. PpGppcan be effectively detected, a baseline is stable, a sampling amount is little, time and cost are saved, gradient elution does not need to be set, the operation is simple and convenient, and the detection effects are good.

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