The invention relates to a method for removing the toxicity of pig serum to passage cell growth, belongs to the technical field of cell culture, and aims to solve the technical problem of pig serum cell toxicity in porcine epizootic diarrhea antiserum. The method includes the steps of firstly, centrifuging pig blood under a sterile condition, and collecting pig serum after the centrifuging; secondly, sealing the pig serum obtained the first step into a sterile container, and then performing inactivation; thirdly, using sterilized activated carbon to adsorb the inactivated pig serum, and then collecting the pig serum; fourthly, subjecting the pig serum obtained after the adsorption to filtering and sterilizing, and then adding into the passage cells which fully grow on a single layer to continue cell culture. The method is applicable to experiments and researches related to cell culture.