The invention provides a pepsinogen I/pepsinogen II detection kit, and belongs to the technical field of fluorescence immunochromatography in medical immunology. The pepsinogen I/pepsinogen II detection kitwhich is a detection kit of fluorescence quantitative immunochromatography, comprises a detection card, and is characterized in that the detection card is provided with a PVC board, a sample pad, a combination pad, a chromatography membrane and a water absorption paper in sequence; and the chromatographic membrane comprises a detection region and a quality control region, a detection regionof an anti-PG I monoclonal antibody and a PG II monoclonal antibody are coated with the detection region, and a sheep anti-mouse IgG antibody is coated with the quality control region. Test samples ofthe reagent kit are human serum, plasma or whole blood samples, and good detection specificity, higher sensitivity, simple and convenient operation and stable fluorescent markers are achieved to ensure the accuracy of the detection.