The invention provides a chuzhou chrysanthemum polysaccharide biscuit and a preparation method thereof, and relates to the technical field of cake processing. The chuzhou chrysanthemum polysaccharidebiscuit comprises, by weight, 50-60 parts of black wheat flour, 10-12 parts of composite juice, 15-20 parts of milk, 6-10 parts of chuzhou chrysanthemum, 2-3 parts of egg white, 0.5-0.8 part of streptococcus lactis, 0.8-1 part of lactobacillus bulgaricus, 5-6 parts of theanine, 0.001-0.1 part of sodium bicarbonate, 2-4 parts of papain, 0.8-1.2 parts of vanilla powder, 2-3 parts of salt and 5-7 parts of butter. The preparation method mainly comprises the steps of crushing and sieving, extracting chuzhou chrysanthemum polysaccharide, stirring and quickly boiling, preserving heat and fermenting,mixing and kneading dough, coating and pressing, baking and forming and the like. By the adoption of the chuzhou chrysanthemum polysaccharide biscuit and the preparation method thereof, the defects ofthe prior art are overcome; the black wheat flour is used as a main raw material, and meanwhile the chuzhou chrysanthemum polysaccharide and other auxiliary materials are added, so that the biscuit with a health-care property is developed; and the product also has good mouthfeel and extremely high nutritional value, and is suitable for popularization and production.