The invention discloses a refining method for 7-ketolithocholic acid. The refining method comprises the following steps that a crude 7-ketolithocholic acid product and alcohol react under acid catalysis to form ester, a crude 7-ketolithocholic acid ester product is obtained, refined in a mixed solvent and hydrolyzed under the alkaline condition, and the refined 7-ketolithocholic acid product is obtained. The refining method is mild in reaction condition and simple in operation, the reagents used in the method are low in cost and easy to obtain, the cost is low, the purifying effect is obvious,and the method is suitable for industrial production.