The invention discloses a method for determining the influence of different osmotic pressures on the deformation of a uniaxial compression limestone compaction stage. The method comprises the following steps: 1) equally dividing N rock standard samples into four groups; 2) drying each group of rock standard samples; 3) connecting the top of each rock standard sample with a water injection device,and then coating the surface of each rock standard sample with a water stop agent; 4) injecting water into each group of rock standard samples through the water injection device at a pressure rise rate of 4 MPa/min, and keeping for a preset time after the water injection pressure of each group of rock standard samples reaches a design value; (5) drawing the stress-strain curve of the rock; (6) establishing a function expression conforming to the rock deformation characteristic; and (7) determining model parameters, and determining the influence of different osmotic pressures on the deformationof the uniaxial compression limestone compaction stage according to the model parameters. According to the method, the influence of the osmotic pressure on the deformation of the uniaxial compressionlimestone compaction stage can be accurately determined.