The invention discloses a measuring and calculating platform for the installation position of an operating microscope, which is used on a glasses frame and comprises an eye tracker which is arranged on the glasses frame and collecting the watching information of human eyes, a front-end camera which is arranged on the glasses frame and coaxially collects external information seen by human eyes; anda data processing terminal which is used for analyzing the watching position of the user in the actual surgical operation according to the watching information collected by the eye tracker and the external information collected by the front-end camera, and measuring and calculating the optimal mounting position of the glasses frame type surgical microscope on the glasses frame. The invention further discloses a using method of the measuring and calculating platform, the watching position of a user in actual surgical operation can be analyzed, and the best installation position of the customized microscope on the glasses frame in the real surgical environment is determined in a personalized mode.