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发明公开
CN110018249A 一种定性定量分析非酶糖基化血浆白蛋白的方法 无效 - 驳回

一种定性定量分析非酶糖基化血浆白蛋白的方法
摘要：
本发明涉及一种定性定量分析非酶糖基化血浆白蛋白的方法，将血浆白蛋白还原烷基化和胰蛋白酶酶解后，通过“纳升液相色谱-多级质谱(nL-MSn)结合“同位素标记的相对定量和绝对定量技术(iTRAQ)”定性定量分析血浆白蛋白非酶糖基化。通过还原烷基化及胰蛋白酶酶解将血浆白蛋白降解成肽段，通过纳升液相分离后再通过高分辨率质谱获取氨基酸序列、糖基化位点信息，并通过iTRAQ报告离子实现糖基化位点的准确定量。本发明实现了对血浆白蛋白糖基化位点全面的定性和定量分析，可用于监测血糖浓度和早期糖尿病的检测。
摘要（英）：

The invention relates to a method for qualitative and quantitative analysis of non-enzymatic glycosylated plasma albumin. After reductive alkylation of plasma albumin and trypsin enzymolysis, non-enzymatic glycosylation of the plasma albumin is analyzed qualitatively and quantitatively by nanoliter liquid chromatography-multistage mass spectrometry (nL-MSn) and isobaric tags for relative and absolute quantitation (iTRAQ). The plasma albumin is degraded into peptide fragments by reductive alkylation and trypsin enzymolysis, amino acid sequence and glycosylation site information are obtained byhigh-resolution mass spectrometry after nanoliter liquid phase separation, and accurate quantification of the glycosylation site is achieved by iTRAQ reporter ions. The invention realizes the comprehensive qualitative and quantitative analysis of the glycosylation site of plasma albumin and can be used for the monitoring of blood glucose concentration and the detection of early diabetes.

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