The invention discloses a carbon quantum dot organic silicon resin with regulatable fluorescence radiation, a preparation method and an application thereof. The preparation method comprises the following steps: taking and uniformly mixing gamma-aminopropyl methyl diethoxy silane, vinyl methyl dimethoxy silane, potassium hydroxide, dimethyl sulfoxide and pure water, vacuumizing, introducing nitrogen, reacting while stirring, and performing rotary evaporation, thereby acquiring polysiloxane; adopting a hydrothermal method for in situ synthesizing polysiloxane modified carbon quantum dot; purifying the carbon quantum dot, thereby acquiring the carbon quantum dot organic silicon resin with regulatable solid fluorescence radiation. According to the invention, abundant carbon quantum dot products can be acquired, wavelength is adjustable and the carbon quantum dot organic silicon resin has excellent solubility in common solvents; due to the modification function of organic silicon, the phenomenon of quantum dot agglomeration is not obvious, the fluorescence stability is high and the photobleaching property under ultraviolet high-energy beam is excellent; the synthetic method is simple, green and environment-friendly; the raw materials are low in cost; luminous efficiency is high and stability is high; the raw materials are low in toxicity, green and environment-friendly; the requirement for processing equipment is low.