Provided in the invention is a detection conversion box of a lateral flow chromatography test strip. The detection box comprises two parts. A test strip box bottom A is independently or movably connected to a test strip box cover B. Channels are arranged at the two sides of the test strip box bottom A; and the width of the test strip box cover B matches the bottom of the box. According to the invention, a tool is provided for splicing of a plurality of test strips; and a defect of manual dismounting is overcome. The box has a reasonably designed structure; and a connection channel is providedfor the test strip by two bar channels. With a stopping block at the top of the cover, the connected test strips is stabilized. The detection conversion box has advantages of compact and light structure, simple process, low cost, and high repeatability; and the box can be abandoned or recycled in case of any special requirement.