The invention relates to a multilayer catalysis layer alleviating attenuation of an alloy contained catalysis layer and a preparation method of the multilayer catalysis layer. The catalysis layer of amultiplayer structure necessarily includes a layer prepared from a non alloy catalyst and a layer prepared from an alloy catalyst, the catalyst layer prepared from the non alloy catalyst is preparedin the side close to a proton exchange membrane, and the alloy catalyst included catalysis layer is prepared in the side close to a gas diffusion layer. Compared with the prior art, the catalyst layerwith a pollution source contained alloy catalyst is far from the proton exchange membrane and close to the gas diffusion layer, and the condition that transitional metal elements are dissolved and pollute the proton exchange membrane can be alleviated.