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发明公开
CN110088297A 锁式探针检测方法 审中-实审

锁式探针检测方法
摘要：
本发明涉及使用两个或更多个锁式探针检测样品中的分析物的多路复用方法，锁式探针各自特异于不同的靶序列，靶序列是分析物的一部分或指示样品中分析物的存在，其中每个锁式探针包含分析物特异性报告序列，以及位于分析物特异性报告序列3’的限制性切割位点，和/或用于扩增反应的第一扩增引物结合位点，其中在锁式探针包含限制性切割位点的情况下，在所述限制性切割位点处的切割发生在分析物特异性报告序列的3’，并且在锁式探针包含用于进一步扩增反应的第一扩增引物结合位点的情况下，锁式探针不包含分析物特异性报告序列的第二扩增引物结合位点5’，本发明还涉及探针的组合和探针的组合的试剂盒。
摘要（英）：

The present invention relates to multiplexed methods of detecting an analyte in a sample using two or more padlock probes each specific to a different target sequence, the target sequence being eitherpart of an analyte or indicative of the presence of an analyte in the sample, wherein each padlock probe comprises an analyte-specific reporter sequence, and either a restriction cleavage site located 3' of the analyte-specific reporter sequence, and/or a first amplification primer binding site for an amplification reaction, wherein where the padlock probe comprises a restriction cleavage site, cleavage at said restriction cleavage site occurs 3' of the analyte-specific reporter sequence, and where the padlock probe comprises a first amplification primer binding site for the further amplification reaction, it does not contain a second amplification primer binding site 5' of the analyte-specific reporter sequence, and panels of probes and kits for the same.

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