The invention discloses an armillaria luteo-virens cell culture device and a culture method based on the device. The device comprises a culture platform, a supporting mechanism, a power mechanism anda fixed disc. The culture platform is arranged on the supporting mechanism, the culture platform is rotationally connected with the supporting mechanism, the power mechanism is arranged on the supporting mechanism, the power mechanism drives the culture platform to rotate, and the fixed disc is arranged on the culture platform. The culture method comprises the following steps that a) the cell culture device is assembled; b) the cell culture device is adjusted; c) a culture medium is prepared; d) a growth regulator is prepared; e) stock culture is carried out. According to the armillaria luteo-virens cell culture device and the culture method, by rotating the culture platform, the difference between the illumination intensities, temperatures and humidities of different plates on the cultureplatform is reduced, the culture speed of armillaria luteo-virens cells is increased, and the growth speed of armillaria luteo-virens is increased.