The invention relates to a split and current collecting interactive assembly manufacturing method for LNG marine polyurethane insulation box; and the method comprises the following steps of: a code scanning process, a loading process, an automatic gluing process, a bottom component assembly process, a wood splint assembly gluing process, a wood plywood assembly process, an underlayer tensioner assembly process, an upper component assembly process, an upper tensioner assembly process, an overall calibration process, a plywood correction process, a top film coating process and a post-treatment process. Compared with the prior art, the invention makes full use of the existing automation equipment, adopts the split and current collecting interactive assembly manufacturing ideas, sets the parallel material transportation mode of the double conveying line, and the multi-station segment assembly method; the influence of the single linear arrangement on the production system due to equipment failure is greatly reduced; the manufacturing method not only reduces equipment investment and the occupied area of a production site, but also effectively balances the operation time of each process,improves production efficiency, and ensures high-precision quality requirement.