The invention discloses a turnover type automatic micro-fluid metering device, and belongs to the technical field of fluid measurement. The device comprises a turnover mechanism and a metering mechanism; the turnover mechanism comprises a turnover groove and a bearing shaft hinged to the bottom of the turnover groove; the turnover groove comprises a first liquid storage tank and a second liquid storage tank which are isolated from each other; the turnover groove is turned over towards the first liquid storage tank side or the second liquid storage tank side by taking the bearing shaft as a supporting point, so that the first liquid storage tank and the second liquid storage tank alternately collect a fluid to be metered; when the turnover groove is turned over each time, the fluid in the liquid storage tank corresponding to the turnover side is discharged; the metering mechanism comprises a weighing module and a monitoring system; the bearing shaft is supported on the weighing module;the weighing module is used for measuring the mass transmitted by the bearing shaft and transmitting measurement data to the monitoring system; and the monitoring system conducts fluid metering according to the measurement data and outputs and displays a fluid metering result. The device has the advantages that an evaporation amount error in fluid metering is reduced; the range limitation is avoided; and the metering precision is high.