The invention provides an improved preparing method of cis-counterpoint substituted cyclohexyl amino nitrile maleate shown in the formula (I). The method includes the steps of adding 0.3-0.55 molar fold of maleic acid to an organic solvent solution of a cis/trans-counterpoint substituted cyclohexyl amino nitrile compound corresponding to the formula (I) for stirring and ultrasonic treatment, and conducting filtering to obtain the cis-counterpoint substituted cyclohexyl amino nitrile maleate shown in the formula (I). In the formula (I), R1 represents a C1-C10 alkyl group or alkyl oxygroup, a C1-C10 vinyl group or vinyl oxygroup, a C1-C10 alkynyl group or alkynyl oxygroup, a C1-C10 naphthenic group or naphthenic oxygroup and a C1-C10 heterocyclic alkyl group or heterocyclic alkyl oxygroup with one or two heteroatoms selected from O and N, and HX represents maleic acid.