The invention discloses a distiller's grain-straw bio-organic fertilizer special for tobacco and a production method thereof. Fermentation materials of the distiller's grain-straw bio-organic fertilizer comprise 40-80 parts of distiller's grains, 20-40 parts of straw, 1.0-2.5 parts of quicklime, 1.0-5.0 parts of rapeseed cake, 2-12 parts of bone meal, 0.1-1.0 part of potassium sulfate and 5.0-10.0parts of a solid microbial agent. The method for producing the distiller's grain organic fertilizer has the advantages that the fermentation time is short, the fermentation degree is controllable, the problems of low potassium content and low C/N ratio of existing distiller's grain organic fertilizers in the prior art are solved, the indexes of a product prepared from the distiller's grain-straworganic fertilizer obtained by means of the method accord with NY884-2012, and the nitrogen-phosphorus-potassium ratio is 4:3:6 which is required by the growth of the tobacco.