The invention provides a microbial enzyme composite preparation and a production method and application in treating industrial sewage or landfill leachate thereof, and relates to the technical field of sewage treatment. The microbial enzyme composite preparation comprises an EM microbial agent, aerobic bacteria protease and anaerobic bacteria protease. According to the microbial enzyme composite preparation and the production method and application in treating the industrial sewage or the landfill leachate thereof, the aerobic bacteria protease and the anaerobic bacteria protease which are extracted from active sludge can provide a large number of active enzymes for aerobic microorganisms and anaerobic microorganisms in the EM microbial agent, the initial reaction speed of the aerobic microorganisms and the anaerobic microorganisms is improved, thus the initial COD removal rate of the EM microbial agent can be significantly improved, and a sewage environment which is likely to upset abalance of the EM microbial agent is fast improved so that the provided microbial enzyme composite preparation can be suitable for treating the various industrial sewage or landfill leachate.