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发明公开
CN110373336A 淡紫拟青霉液体深层发酵生产孢子的发酵培养基及方法 审中-实审

淡紫拟青霉液体深层发酵生产孢子的发酵培养基及方法
摘要：
本发明提供一种淡紫拟青霉液体深层发酵生产孢子的发酵培养基及其生产方法。所述培养基由含有以下配比的物质：蔗糖或葡萄糖45-80g/L、可溶性淀粉23-40g/L，大豆粉或棉籽粉20-35g/L，磷酸二氢钾2-4g/L，无水氯化钙0.1-0.4g/L，七水合硫酸镁0.1-0.3g/L，六水合氯化钴0.01-0.037g/L，七水合硫酸铁0.01-0.05g/L，一水合硫酸锰0.005-0.016g/L，七水合硫酸锌0.005-0.014g/L。本发明液体深层发酵的发酵时间为55-65h，温度为26-28℃，溶氧控制采用两段控制，0-24h菌体生长阶段，溶氧值控制为45-60%，24-65h孢子形成及成熟阶段，溶氧值控制为10-15%。本发明显著地缩短了淡紫拟青霉深层发酵产孢子的发酵周期，大幅度增加了产孢量，降低淡紫拟青霉的发酵成本，所生产出的孢子具有很好的杀根结线虫的能力。
摘要（英）：

The invention provides a fermenting medium for producing spores by liquid submerged fermentation of Purpureocillium lilacinum and a production method of the fermenting medium. The fermenting medium comprises 45-80 g/L of sucrose or glucose, 23-40 g/L of soluble starch, 20-35 g/L of soybean flour or cottonseed powder, 2-4 g/L of monopotassium phosphate, 0.1-0.4 g/L of anhydrous calcium chloride, 0.1-0.3 g/L of magnesium sulfate heptahydrate, 0.01-0.037 g/L of cobalt chloride hexahydrate, 0.01-0.05 g/L of iron sulfate heptahydrate, 0.005-0.016 g/L of manganese sulfate monohydrate, and 0.005-0.014 g/L of zinc sulfate heptahydrate. Fermenting time of the liquid submerged fermentation is 55-65 h; fermenting temperature is 26-28 DEG C; dissolved oxygen control is subjected to two-stage control;dissolved oxygen value is controlled to 45-60% in the 0-24-hour bacterial growth stage of 0-24 hours, and the dissolved oxygen value is controlled to 10-15% in the 24-65-hour spore formation and maturing stage. The fermenting cycle for producing spores by liquid submerged fermentation of Purpureocillium lilacinum is shortened evidently; spore yield is increased greatly; fermentation cost of Purpureocillium lilacinum is reduced; the produced spores can well kill root-knot nematodes.

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