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发明公开
CN110373414A 一种高效创制磺酰脲类除草剂抗性玉米的方法及其应用 审中-公开

一种高效创制磺酰脲类除草剂抗性玉米的方法及其应用
摘要：
本发明涉及植物基因工程技术领域，具体涉及一种高效创制磺酰脲类除草剂抗性玉米的方法及其应用。本发明提供特异性靶向玉米ALS1和ALS2基因的sgRNA、含有该sgRNA的CRISPR/Cas9基因组单碱基编辑载体，以及利用该载体进行精确目标DNA定点替换突变创制磺酰脲类除草剂抗性玉米种质的方法。本发明提供的单碱基编辑载体能够实现较高的ALS1和ALS2基因的单碱基突变效率，显著提高了ALS1和ALS2基因定点纯合替换以及磺酰脲类除草剂抗性玉米制备的效率，制备得到的磺酰脲类除草剂抗性玉米可耐受300mg/L的氯磺隆除草剂喷施处理，具有很高的育种和栽培价值。
摘要（英）：

The invention relates to the technical field of plant gene engineering, in particular to a method for efficiently preparing corn resistant to sulfonylurea herbicides and application of the method. AnsgRNA for specific targeting of ALS1 and ALS2 genes of the corn, a CRISPR/Cas9 genome single-base editing carrier containing the sgRNA and a method for utilizing the carrier for conducting precise target DNA fixed-point substitution and mutation to prepare a corn germplasm resistant to the sulfonylurea herbicides. By means of the single-base editing carrier, high single-base mutation efficiency ofthe ALS1 and ALS2 genes can be achieved, the efficiency of fixed-point homozygosis substitution of the ALS1 and ALS2 genes and the efficiency of preparation of the corn resistant to the sulfonylureaherbicides are significantly improved, and the prepared corn resistant to the sulfonylurea herbicides can be tolerant to spraying treatment of a 300 mg/L chlorsulfuron herbicide, and has high breedingand cultivation value.

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