The invention discloses an identification method of space non-cooperative target spinning parameter using an image sequence. The identification method comprises the following steps: firstly shooting an image of a target spacecraft, secondly, performing characteristic peak detection processing on each obtained image to obtain an image coordinate of a sailboard, and then inversely solving possible attitudes of the target spacecraft according to the obtained peak image coordinate of the sailboard, and finally fitting the attitude sequences obtained by processing multiple images to obtain a spin axis and rotating speed. The method disclosed by the invention is suitable for the non-cooperative target with less prior geometrical information (only needing the length-width ratio of the characteristic), and is limited to a condition that the sailboard and the body are free from relative rotation.