The invention relates to an experimental device and method for inhibiting an optical frequency shift virtual magnetic field of an SERF magnetometer. Due to combination of an optical fibre beam splitter and a wavemeter, the centre frequency of optical frequency shift can be measured by adjusting or setting the pumping light frequency for many times; then, pumping light is tuned to the centre frequency, so that optical frequency shift and a magnetic field generated by optical frequency shift are eliminated; and thus, the problem that work of the magnetometer is influenced by the optical frequency shift virtual magnetic field is avoided.