The invention provides Mozzarella cheese and a preparation method thereof. The raw materials of the Mozzarella cheese comprise raw milk, a compounding fermenting agent and a stabilizing agent, whereinthe compounding fermenting agent comprises a first fermenting agent and a second fermenting agent, the first fermenting agent is selected from streptococcus thermophilus, and the second fermenting agent is selected from one or more of lactobacillus cremoris, lactococcus lactis subsp lactis, lactococcus lactis subsp. cremoris, streptococcus diacetylactis and leukonoid. The Mozzarella cheese disclosed by the invention is uniform in melt properties, good in wire drawing effects, high in oil yield after being heated, small in quantity of formed burned marks, good in flavor and mouth feel, and high in stability within the quality guarantee period, the preparation method is short in time consumption and simple and convenient to operate, and the Mozzarella cheese is suitable for large-scale production.