The invention provides a facial mask with a placenta mesenchymal stem cell culture supernate and a preparation method of the facial mask and relates to the field of daily cosmetics. The facial mask isprepared from the placenta mesenchymal stem cell culture supernate, a witch hazel extraction solution, a buckwheat seed extract, a humectant, a water retaining agent, a thickening agent, an emulgator, vitamins, collagen and chitosan. The facial mask has the advantages that the epidermal cells are deeply nourished, cell repairing and regeneration are promoted, and skin healing is promoted.