The invention relates to the technical field of nano delivery, in particular to a novel HA-SP conjugate. The conjugate is characterized by being prepared by the following steps: dissolving HA into PBS, adding EDC and NHS with stirring, adding dopamine into the obtained solution, dissolving diethylamine into PBS, dropwise adding the diethylamine, adjusting the pH value to 4, performing reaction for48 hours under the protection of nitrogen, performing dialyzing, and performing freeze-drying to obtain a product HA-DN-EA; dissolving SP in MC, and performing stirring in an ice bath; dropwise adding TEA into the obtained solution, performing reaction for 24 hours under the protection of nitrogen, placing the reacted materials in a dark place at room temperature, and performing separating to obtain a closed-loop SP; and mixing the HA-DN-EA and the SP in DMSO (dimethylsulfoxide), performing reaction for 48 hours in the dark place at room temperature, performing dialyzing after the reaction isfinished, and performing freeze-drying to obtain the HA-SP conjugate. According to the invention, the conjugate penetrates the skin and stays in the corium layer through nano-effect, so that the medicine is retained in the skin and is continuously released.