The invention relates to a method for high-yield bacterial strains by high-flux screening of gentamycin based on an ARTP composite mutagenesis technique. The method comprises the steps of preparing aspore suspension, performing ARTP mutagenesis composite mutagenesis, performing quick detection on products, prescreening high-yield bacterial strains with gentamycin and performing screening once again to obtain high-yield bacterial strains and performing stability inspection on the high-yield bacterial strains. Compared with the prior art, the method has the advantages that the principle that OPA and the gentamycin are subjected to a reaction to form characteristic absorption peaks of about 330nm is adopted, through a microplate reader, quick determination of an OD value is realized, so thatfast detection of the gentamycin can be successfully realized. The interference problems that poor specificity of a phosphotungstic acid method and a method for forming precipitate with the gentamycin to cause reduction of the OD value, fermentation liquid color and the like, are solved. The method has the characteristics of being quick, high-flux, convenient to operate, accurate and the like, and the mutation breeding and screening efficiency of the gentamycin is improved to a great extent, so that the probability for obtaining the high-yield bacterial strain with the gentamycin is greatly improved.