The invention discloses a bacterial drug resistance electrochemical detection method and belongs to the fields of electrochemical chips and bacterial detection technologies. According to the method, aspecific sample solution containing resazurin is utilized to perform drug sensitivity detection on bacteria in combination with differential pulse voltammetry in a specific electrochemical environment. Through the method, bacterial activity can be directly detected, accuracy is high, operation is easy, determination of drug resistance and minimal inhibitory concentration can be performed in a short time, and the method can be widely applied to all kinds of research and clinical detection in the medical field and other fields.