The invention discloses a method for simultaneously analyzing nine alkaloids and 11 aroma components in tobacco leaf. The method for simultaneously analyzing nine alkaloids and 11 aroma components inthe tobacco leaf comprises the steps of sample pre-treatment and standard curve quantitative analysis, migrating the target alkaloids and the aroma substances away from a sample matrix through alkaliliquor soaking, and adding trichloromethane, performing vortex oscillation, and transferring the alkaloids and the aroma substances into a trichloromethane phase, taking the trichloromethane phase toperform instrument analysis after performing centrifugal stratification. Compared with the prior art, the method disclosed by the invention has the advantages that the method for simultaneously analyzing nine alkaloids and 11 aroma components in tobacco leaf has not been reported at present; compared with the existing alkaloid and aroma component analysis method of the tobacco leaf, the method disclosed by the invention is simpler in operation and higher in stability.