The invention belongs to the field of genetic engineering, and more specifically discloses recombinant human fibronectin peptide. The invention discloses a recombinant human fibronectin peptide gene after codon optimization, and by a genetic engineering method, expression and purification are performed in vitro. Escherichia coli is used as an expression host cell, and the recombinant human fibronectin peptide which is small in molecular weight, reserves original characteristics of fibronectin and can promote cell growth, increase cell adherence rate and reinforce cell metabolism level is provided.