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发明公开
CN110592029A 利用青枯菌复杂种性扩繁烟草青枯菌噬菌体的方法 无效 - 驳回

利用青枯菌复杂种性扩繁烟草青枯菌噬菌体的方法
摘要：
本发明公开了一种利用青枯菌复杂种性扩繁烟草青枯菌噬菌体的方法，包括：(1)挑选青枯菌噬菌体，所述青枯菌噬菌体既能够裂解宿主为烟草的青枯菌，又能够裂解宿主为其它作物的青枯菌；(2)选择扩繁宿主，采用其它作物的青枯菌作为扩繁烟草青枯菌噬菌体的宿主；(3)利用青枯菌复杂种性扩繁烟草青枯菌噬菌体，将所述青枯菌加入到CPG液体培养基中震荡培养，当吸光值OD600为0.2-0.3时，加入所述青枯菌噬菌体菌液震荡培养，离心取上清液过滤获得扩繁后的噬菌体溶液备用。本发明可以降低在扩繁过程中烟草青枯菌对噬菌体产生抗性的风险，也避免了在烟草青枯菌噬菌体菌剂等产品中携带有少量未被裂解烟草青枯菌带来的潜在风险。
摘要（英）：

The invention discloses a method for propagating pseudomonas solanacearum bacteriophage by utilizing complex characteristics of ralstonia solanacearum. The method comprises the following steps: (1) selecting a pseudomonas solanacearum bacteriophage which can lyse ralstonia solanacearum taking tobaccos as a host or ralstonia solanacearum taking other crops as a host; (2) selecting a propagation host, namely adopting ralstonia solanacearum of other crops as the host for propagating the pseudomonas solanacearum bacteriophage; and (3) propagating the pseudomonas solanacearum bacteriophage by utilizing the complex characteristics of ralstonia solanacearum, namely adding the ralstonia solanacearum into a CPG liquid medium, performing shake culturing, adding a bacterial solution of the ralstoniasolanacearum bacteriophage into the CPG liquid medium and performing shake culturing when an optical density OD600 is 0.2-0.3, performing centrifuging to obtain supernate, and performing filtering toobtain a propagated bacteriophage solution for later use. The method can be used for reducing the risk that pseudomonas solanacearum generates resistance on bacteriophage in a propagating process, andcan avoid a potential risk caused by a small amount of not lysed pseudomonas solanacearum carried in pseudomonas solanacearum bacteriophage microbial inoculums and other products.

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