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发明公开
CN110646372A 一种类黑精的定量检测方法 无效 - 驳回

一种类黑精的定量检测方法
摘要：
本发明公开了一种类黑精的定量检测方法，属于检测技术领域。本发明采用模拟类黑精标品，通过标准曲线来定量检测待测样品中的类黑精含量；所述模拟类黑精标品是以葡萄糖和氨基酸为原料模拟美拉德反应，以找到与类黑精结构最相近(即红外出峰位置和峰型最为接近)的模拟产物，将其作为类黑精标品。本发明通过采用糖和20种氨基酸分别进行美拉德反应，对生成的20种反应产物和白酒糟类黑精同时进行红外(FT-IR)结构鉴定和对比，以找到与酒糟类黑精结构最相近的模拟产物，将其作为酒糟类黑精标品，通过建立标准曲线完成白酒糟类黑精的定量，这种方法得到的标准曲线呈现良好的线性关系，且精密度和准确性均较好。
摘要（英）：

The invention discloses a quantitative detection method of melanoidins, and belongs to the technical field of detection. The method provided by the invention uses a simulated melanoidins standard to quantitatively detect the melanoidins content in a sample to be measured by a standard curve, and the simulated melanoidins standard uses glucose and amino acids as raw materials to simulate Maillard reaction to find out a simulated product with the most similar structure as the melanoidins (that is, the most similar infrared peak position and peak shape) to be used as the melanoidins standard. Themethod provided by the invention performs the Maillard reaction by using sugar and 20 kinds of amino acids, respectively, synchronously performs infrared (FT-IR) structure identification and comparison on the generated 20 kinds of reaction products and distiller melanoidins to find out the simulated product with the most similar structure as the distiller melanoidins, uses the simulated product as a distiller melanoidins standard, and completes the quantification of the distiller melanoidins by establishing a standard curve. The standard curve obtained by the method shows a good linear relationship, and is good in both precision and accuracy.

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