The invention belongs to the technical field of animal model building and discloses a chronic food-borne Parkinson's disease mouse model and a building method and application thereof. The method includes: a 5% ferric citrate solution is used to continuous intervene a C57BL/6 mouse for 16 weeks, behavioral evaluation is performed through open-field, speed-up rotation and pole climbing experiments every other 4 weeks, and the mouse of the 16th week is subjected to a suspension experiment and a food award type Y-maze experiment; the measured mouse total journey, average speed, center times, center journey, center time and on-rod time of the mouse are evidently reduced as compared with those of the control, turning time and pole climbing time of the mouse are evidently increased as compared with those of the control, the results are time-dependent, and mouse sports coordination, balance ability, muscular tension and activity are lowered evidently. The chronic food-borne Parkinson's diseasemouse model and the building method have the advantages that the occurrence conditions of Parkinson's disease are simulated, and the chronic model is suitable for monitoring early-stage change and screening the early-stage diagnosis indexes of the Parkinson's disease; behavioral disorder can be quantified, and the pathological characteristic of the Parkinson's disease is dopaminergic neuron degeneration and loss characteristic lesion.