The invention belongs to the technical field of chemical detection, and discloses a preparation method of immunomagnetic beads for detecting edetate, and prepared immunomagnetic beads. The preparationmethod of the immunomagnetic beads is as follows: (1) taking magnetic beads, suspending the magnetic beads in a buffer solution after washing the same; (2) adding an edetate monoclonal antibody solution into the magnetic bead suspension, performing oscillation and uniform mixing, performing coupling activation at 10-45 DEG C for 20-120min, then performing stewing and layering on a magnetic forceframe, and discarding the supernatant to obtain a magnetic bead-antibody compound; and (3) adding the magnetic bead-antibody compound into the buffer solution to suspend the same, adding confining liquid, performing incubation at room temperature, and performing stewing and layering on the magnetic force frame to obtain the immunomagnetic beads. The immunomagnetic beads prepared by the preparationmethod disclosed by the invention have high specificity and high sensitivity, and can quickly and specifically bind to the edetate in a sample solution to be tested, quickly enrich the edetate in thesample to be tested and greatly improve the detection limit and the detection accuracy of the edetate.