The invention provides a preparation method of cross-linking microspheres based on microfluidics. The preparation method comprises the following steps of pushing an injector of a water phase solutionand an oil phase solution which are prepared in advance through a pushing and injecting pump device, enabling a water-oil two-phase solution to form water-in-oil type emulsion liquid drops at an exitchannel of a micro-fluidic chip, and performing illumination at an ultra-violet curing lamp to prepare PEGDA photo-crosslinking microspheres; and enabling the PEGDA photo-crosslinking microspheres tobe charged into a sodium hydroxide solution so as to obtain PEGDA-chitosan cross-linking microspheres, wherein the diameter range of the cross-linking microspheres is 240-440[mu]m, and is controlled through the flow velocity and the flow velocity ratio of the water phase solution and the oil phase solution. The preparation method is flexible and easy to operate. A preparation method of an injecting type chondrocyte composite body comprises the following steps of A1, preparing the cross-linking microspheres; and A2, enabling the cross-linking microspheres to carry chondrocyte. Biological cellsare carried on the cross-linking microspheres having a dual-network hydrogel system, and through proliferation and disintegration of the biological cells, the injecting type cell composite body can beprepared, and is a novel tissue repair material which is widely applied.