The invention provides a display substrate, a preparation method and a display device. The display substrate comprises: a substrate, wherein a plurality of pixel units are limited by grid lines and data lines which are crisscross at one side of the substrate; a grid electrode for receiving a grid electrode driving signal of a corresponding GOA (Gate driven On Array) unit through the grid line of apixel unit row where the grid electrode locates; and an pull-up electrode which is electrically connected with a pull-up node of a GOA unit corresponding to a pixel unit row where the pull-up electrode locates; wherein the pull-up electrode and the grid electrode are separated at least through an insulation layer, an orthographic projection of at least one of the grid electrode and the grid lineon the substrate and an orthographic projection of the pull-up electrode on the substrate have an overlapped part. The preparation method is suitable for preparing the display substrate. According tothe display substrate of the invention, the pull-up electrode is manufactured within an pixel area, so as to form part or whole of a storage capacitance of a GOA circuit with the grid electrode/grid lines in the pixel area, thereby effectively reducing space occupation of the GOA circuit in a non-display area, and facilitating realization of the display substrate with a narrow frame.