The invention relates to a multi-source signal acquisition card. The multi-source signal acquisition card comprises an integrated processor, a first communication interface component, a second communication interface component, each FPGA acquisition module, and each signal switching circuit; the integrated processor is connected to each FPGA acquisition module through the first communication interface component respectively; the FPGA acquisition modules are connected with sensors through the signal switching circuits; the integrated processor is connected with an expansion device through the second communication interface component; the integrated processor communicates with an interface corresponding to the second communication interface component when the FPGA acquisition modules are connected with the first communication interface component; and the integrated processor communicates with the interface corresponding to the second communication interface component when the expansion device is connected with the second communication interface component. When the multi-source signal acquisition card collects multiple signal sources, signal acquisition signal channels can be adaptively switched, distortion of sampling data is avoided, high scalability is achieved, and the acquisition efficiency is improved.