The present invention belongs to the technical field of biological engineering and particularly relates to a method for producing high temperature resistant neutral protease. A production strain usedin the method is specifically bacillus subtilis SJ-215 and has a deposit number of CGMCC No.18645. At the same time, the present invention also optimizes a corresponding fermentation mechanism. The fermentation mechanism has higher fermentation vigor, higher extraction yield, lower manufacturing cost, and average fermentation enzyme activity above 65,000 U/mL. The produced neutral protease is highin enzyme activity, good in thermal stability and stable in performance, and has a wide range of application prospects.