The invention relates to a three-dimensional circuitous biological purification pond. The pond comprises a pond body, at least two laminates and multiple biological fillers, wherein a sewage inlet anda sewage outlet are formed in the pond body; the at least two alternates are arranged in the pond body in a spaced mode in the longitudinal direction and divide the pond body into at least three purification bins, water passing holes are formed between the laminates and pond walls of the pond body in a spaced mode, the water passing holes and the purification bins are arranged alternately one byone in the flowing direction of sewage and sequentially communicate to from an S-shaped purification channel, and the sewage inlet and the sewage outlet communicate with the upstream end and the downstream end of the S-shaped purification channel respectively; and the multiple biological fillers are distributed in each purification bin. According to the pond, the sewage flows through all the purification bins sequentially and can generate one biological purification reaction with the biological fillers in each purification bin, contact reaction time of the sewage with the biological fillers islong, it is guaranteed that sewage purification treatment is thorough, and the purification treatment effectiveness of the purification pond on the sewage is greatly enhanced.