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发明公开
CN110785493A 模块式核酸衔接头 审中-实审

模块式核酸衔接头
摘要：
本公开内容提供了用于制备核酸文库的试剂盒。所述试剂盒包括第一和第二寡核苷酸，其各自具有尾序列、共同序列以及独特标识符序列和可变长度标点标记中的至少一种。所述试剂盒进一步包括第一引物，其具有第一样品标识符序列和在所述第一引物的3’端的第一引发序列。所述第一引发序列包括所述第一寡核苷酸的所述尾序列。所述试剂盒进一步包括第二引物，其具有第二样品标识符序列和在所述第二引物的3’端的第二引发序列。所述第二引发序列与所述第二寡核苷酸的所述第二尾序列互补。
摘要（英）：

The present disclosure provides a kit for preparing a library of nucleic acids. The kit includes first and second oligonucleotide, each having a tail sequence, a common sequence, and at least one of aunique identifier sequence, and a variable length punctuation mark. The kit further includes a first primer having a first sample identifier sequence and a first priming sequence at a 3' end of the first primer. The first priming sequence includes the tail sequence of the first oligonucleotide. The kit further includes a second primer having a second sample identifier sequence and a second priming sequence at a 3' end of the second primer. The second priming sequence is complimentary to the second tail sequence of the second oligonucleotide.

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