The invention discloses a high-temperature-resistant mustard-flavored seasoning and a preparation method and application thereof, and belongs to the technical field of seasoning processing. Accordingto the formula, the high-temperature-resistant mustard-flavored seasoning is prepared from the following components in parts by weight: 15-20 parts of horseradish powder, 5-10 parts of mustard powder,5-10 parts of white vinegar, 2-4 parts of salt, 3-9 parts of xylitol, 50-70 parts of water, 6-8 parts of sunflower oil, 0.1-0.3 part of ginger powder, 0.4-0.8 part of xanthan gum, 0.1-0.4 part of white pepper, 4-6 parts of starch sodium octenylsuccinate, 0.2-0.5 part of nano silica sol, 5-6 parts of beta-cyclodextrin, 0.1-0.2 part of ascorbic acid and 0.2-0.4 part of glyceryl monostearate. The high-temperature-resistant mustard-flavored seasoning is prepared by taking horseradish as a raw material through the steps of freeze drying, ball-milling superfine pulverization, heating dissolving, stirring, soaking, burdening and radio frequency sterilization. The high-temperature-resistant mustard-flavored seasoning can be applied to coated high-temperature fried food or coated high-temperaturebaked food, the problem that allyl isothiocyanate substances do not resist heat and are volatile in the heating process of the mustard-flavored seasoning is solved, and the mustard flavor preservationrate of mustard sauce under the high-temperature condition exceeds 95%.