The invention discloses walnut and perilla frutescens fish-oil capsules having effects of nourishing brain, soothing nerves and strengthening memory and a preparation method of the walnut and perillafrutescens fish-oil capsules having effects of nourishing brain, soothing nerves and strengthening memory. The walnut and perilla frutescens fish-oil capsules having effects of nourishing brain, soothing nerves and strengthening memory comprise the following components in parts by weight of 10-15 parts of walnut oil, 8-10 parts of fish oil, 5-10 parts of perilla frutescens oil, 10-15 parts of pumpkin powder, 5-8 parts of yellow horn oil, 10-12 parts of COJAG, 3-5 parts of American ginseng yeast, 5-8 parts of whole egg powder, 2-4 parts of arachidonic acid, 1-2 parts of gamma-aminobutyric acid,3-5 parts of ammonium glycyrrhizinate, 1-2 parts of spina date seeds and 1-2 parts of stachyose. Various components containing ingredients capable of nourishing the brain and benefitting intelligenceare reasonably compounded and used, so that health-care products which are suitable for teenagers, high-tensile intellectual work staff, mid-aged and aged people and crowds suffering from memory decline, and have the effects of nourishing the brain, benefitting intelligence, tranquilizing mind, soothing the nerves and strengthening memory can be obtained.