The invention discloses a side plate positioning and assembling tool in a wheeled vehicle body assembling and welding production line, belongs to the technical field of wheeled vehicle positioning andassembling, and aims to solve the problems of poor assembling precision and low production efficiency in the prior art. The side plate positioning and assembling tool comprises a pushing and positioning device, a magnetic positioning device and an inclined plane rotation positioning device, wherein the pushing and positioning device comprises at least one bottom plate, a supporting frame and a collision block; the supporting frame is perpendicular to the bottom plate and is in sliding fit with the bottom plate; the collision block is fixed on one side surface of the supporting frame in the perpendicular sliding direction; the magnetic positioning device is arranged on the same side surface of the supporting frame; the magnetic positioning device at least comprises a positioning plate andan electromagnet; the positioning plate is connected with the supporting frame through an angle adjusting device; the electromagnet is arranged on the positioning plate; the angle of the positioning plate relative to the supporting frame is adjusted through the angle adjusting device; the inclined plane rotation positioning device is arranged on one side surface, which is opposite to the supporting frame, of the positioning plate; the inclined plane rotation positioning device and the electromagnet are positioned on two sides of the positioning plate; and the inclined plane rotation positioning device positions the inclined position of the upper part of a side plate.