The present invention discloses a high-grade fatty primary alcohol mixture extracted from beeswax and its separation purification method, belonging to the separation, purification and deep-processingtechnology of beeswax. The described high-grade fatty primary alcohol mixture is the C24-C34 alkylalcohol mixture containing octacosyl alcohol to 60%-90%. The separation purification method of said mixture uses the saponified beeswax as raw material and adopts the molecular distillation process to obtain the invented product. Its technical characteristics are that it adopts the liquid residue as raw material for next distillation, and makes repeated distillation process and definition of operation techonlogical parameters. Said invented method is simple and its yield rate is high.