The present invention belongs to the field of biological product, and relates to a kind of nose drops for inactivating propagative virus of respiratory tract and its preparation method. Said invention contains inactivated new type coronavirus and medicinal application. It adopts the new coronavirus strain separated from nasopharyngeal fluid of early SARS patient to inoculate Vero passage cell, then utilizes formaldehyde to inactivate virus, after inactivation makes blind passage for three generation, and verifies that it has no living virus, then uses immunofluorescence staining to prove that the virus remains antigenicity, and regulates its concentration and pH value, so that said nose drops can be used for preventing SARS.