The invention relates to a bionic culture method for winter worm summer herb mycelium, which comprises: separating and pure culturing monospore, preparing liquid medium, inoculating the pure culture bacterium of mycelium, static culturing the superficial-layer liquid for six months; harvesting the mycelium; wherein, breaking the mycelium into monocellular or multicellular mycelium every month during the culture process. This invention needs no fermentor or other dear devices, and reduces cost more than 50% for 1g dry mycelium.